Fig. 2.

Mature TGF-β1 and LAP compete with VEGF for binding to Nrp1-Fc. (A) TGF-β1 and free LAP but not BSA-reduced binding of VEGF (2 nM) to the plate coated with 1 nM Nrp1-Fc. (B) Premixing LAP-TGF-β with VEGF (1 nM each) decreased the retention of VEGF by the Nrp1-Fc-coated plate. Under the same conditions, IFN-γ did not affect the VEGF retention. (C) Immobilized LAP C-terminal peptide, QSSRHRR, but not control peptide, bound soluble Nrp1-Fc. (D) Soluble QSSRHRR peptide reduced binding of LAP, LAP-TGF-β1, and VEGF to immobilized Nrp1-Fc (1 nM). The data in A–D are representative of two or three independent experiments.