Table 2.
structural segment |
xu (±SD) (nm) |
ku (±SD) (s−1) |
ΔGu‡ (kBT) |
K (N/m) |
---|---|---|---|---|
α-helix G | 0.16 ± 0.04 | 4.7 ± 8.9 × 10−1 | 21 | 6.9 |
loop GF | 0.15 ± 0.02 | 1.8 ± 1.7 | 20 | 7.3 |
loop GF and α-helix F |
0.42 ± 0.32 | 7.9 ± 53.6 × 10−2 | 23 | 1.1 |
α-helix F | 0.32 ± 0.05 | 3.1 ± 3.2 × 10−1 | 22 | 1.8 |
α-helices E and D |
0.33 ± 0.02 | 9.8 ± 7.4 × 10−3 | 25 | 1.9 |
α-helix E | 0.44 ± 0.07 | 2.3 ± 5.3 × 10−4 | 29 | 1.2 |
α-helix D | 0.58 ± 0.19 | 2.1 ± 9.4 × 10−4 | 29 | 0.7 |
α-helices B and C |
0.69 ± 0.18 | 4.2 ± 13.6 × 10−4 | 28 | 0.5 |
α-helix C | 0.39 ± 0.05 | 5.9 ± 5.1 × 10−2 | 24 | 1.3 |
α-helix C and loop BC |
0.38 ± 0.09 | 7.3 ± 13.4 × 10−2 | 23 | 1.3 |
loop BC and α-helix B |
0.37 ± 0.04 | 9.1 ± 7.7 × 10−2 | 23 | 1.4 |
loop BC | 0.61 ± 0.18 | 1.4 ± 4.6 × 10−3 | 27 | 0.6 |
α-helix B | 0.48 ± 0.07 | 6.6 ± 6.8 × 10−2 | 23 | 0.8 |
α-helix A (and N terminus) |
0.68 ± 0.1 | 1.9 ± 3.6 × 10−4 | 29 | 0.5 |
Values shown for parameters xu and ku, obtained from DFS experiments26 indicate structural and energetic properties of each structural segment.