Fig. 1.

NIKΔT3 expression strongly induces the alternative NF-κB pathway. (A) NIK or NIKΔT3 are expressed under control of the ROSA26 promoter after Cre-mediated deletion of the loxP-flanked STOP cassette. Flag-tagged wild-type NIK and NIKΔT3, which lacks the T3BD (amino acids 78–84) were used for this approach. KD, kinase domain. (B) B cell-specific expression of the IRES-eGFP-containing construct can be verified by FACS analysis. (C–H) Western blot analysis of extracts from control, NIKtg, and NIKΔT3tg B cells, as indicated above the individual blots. The levels of β-actin, tubulin, and lamin B1 are shown as loading controls for whole-cell, cytoplasmic (C), and nuclear (N) extracts, respectively. Numbers in red indicate average quantifications (normalized to the respective loading controls) relative to controls of four (D), five (E), three to six (F), four (G), or three to four (H) experiments.