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Assay and measurement of intramolecular excision events. (A) After quenching UNG with a potent and specific oligonucleotide inhibitor, the single- and double-excision products are digested to discrete dsDNA fragments by using abasic endonuclease and a nicking restriction enzyme. (B) Time course for excision of uracils from S20. (C) Time course for excision of uracils from S400. Reactions were at 37°C with 10 mM NaCl.
