Table 1.

Transfer of [³H]octanoyl from [³H]octanoyl CoA to GSSFL-NH₂, a pentapeptide corresponding to the first five amino acids of ghrelin

Additions to assay					[3H]Octanoyl transferred, fmol per tube
Tube	Membranes	Wild-type GSSFL-NH2	Mutant GSAFL-NH2	Wild-type proghrelin	Experiment A	Experiment B
1	−	+	−		18	14
2	+	−	−		28	20
3	+	+	−		171	209
4	+	−	+		23	28
5	+			−	8
6	+			+	310

Each 50-μl reaction mixture contained 50 μM palmitoyl CoA, 1 μM [³H]octanoyl CoA (11 dpm/fmol), and (where indicated) 50 μg membrane protein, 500 μM of the indicated peptide, or 8 μM proghrelin-His8. After incubation at 37°C for 10 min, each reaction mixture was subjected to either TLC analysis (tubes 1–4) or nickel chromatography assay (tubes 5 and 6) as described in SI Experimental Procedures. The complete TLC profile for Exp. A (tubes 1–4) is shown in Fig. S1.