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. 2008 Jul 31;105(31):10859–10864. doi: 10.1073/pnas.0800647105

Fig. 5.

Fig. 5.

Binding of ZBTB20 to the AFP promoter. (A) ZBTB20 bound to fragment −108/−53 of the Afp gene to form a DNA-protein complex. (B) The DNA-protein complex formed by labeled Afp −108/−53 and ZBTB20 was blocked by excessive unlabeled probe and supershifted by anti-ZBTB20 antibody. (C) ChIP analysis of normal mouse liver at the age of 2 months. Conventional PCR analysis was performed to determine the association of ZBTB20 with the promoter region of AFP in comparison to mouse 18S ORF (ORF). H3 antibody is shown as an internal control for chromatin recovery. IgG antibody is shown as a negative control. (D) Real-time PCR was performed to quantify antibody-bound DNA fragments of multiple liver ChIPs. The graph represents fold enrichment of ZBTB20 bound at AFP promoter region compared with 18S ORF. All values generated by real-time PCR analysis were normalized to control IgG precipitations. Each bar represents the average result from two independent ChIP experiments and six real-time PCR analyses.