Sequence of Native American and Chamorro JCV type 2A compared with previously defined types and subtypes in the VP1 typing fragment. These sites are in the 215-bp fragment amplified by primers JTP-15 and JTP-16. types 1 and 2 are distinguished at sites 1771, 1818, and 1870 (bold). Subtyping sites are located at positions 1843 and 1850 (type 1), or at 1837 and 1850 (type 2) (italic). type 2C is defined by “T” and “G” at these subtyping sites, but may not be an independent group. The large majority of Navaho (41/46), Flathead (9/16), and Chamorro (17/20) strains are type 2A. All 17 Chamorro type 2A strains have a variant “T” at position no. 1805. The invariant positions listed here define types 3, 4, and 6 (not shown). H = A, T, or C; Y = C or T.