Fig. 5.
Mouse striatal cells expressing mutant Htt have fewer P bodies and show reduced silencing activity. (A) Indirect immunofluorescence of mouse striatal cells expressing WT Htt (STHdhQ7/Q7) or mutant Htt (STHdhQ111/Q111) probed with antibodies to Dcp1a. (B) Quantification of the number of P bodies per cell performed on 100 cells. P value (paired t test) <0.001 (C) siRNA reporter assay was performed as described for Fig. 4A. The graph compares relative repression ratios in cells expressing WT (7Q/7Q) or mutant (111Q/111Q) Htt (n = 9, Left; n = 12, Right). (D) Immunoblot analysis of cell lysates prepared for the assay shown in C.