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. 2008 Jul 29;105(31):10744–10749. doi: 10.1073/pnas.0804396105

Fig. 3.

Fig. 3.

Pharmacological profile of mutant Rant21. Saturation binding of (A) agonist and (B) antagonist to solubilized receptors: wild-type A2aR, filled squares; Rant21, open circles. (C–F) Inhibition of [3H]-ZM241385 binding by increasing concentrations of the antagonists XAC (C) and theophylline (D) and the agonists NECA (E) and RPIA (F); wild-type A2aR, filled squares; Rant21, open circles. Binding of [3H]-ZM241385 (10 nM) in the absence of unlabeled ligand was set to 100%. Each solubilized receptor was incubated with ligands for 1 h on ice in binding buffer (50 mM Tris, pH 7.5, and 0.025% DDM) either in the presence (400 mM; A, C–F) or absence of NaCl (B). Data shown are from two independent experiments with each data point measured in triplicate. KD and Ki values are given in Tables 1 and S4.