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. 2008 Jul 31;105(31):10815–10819. doi: 10.1073/pnas.0804721105

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© 2008 by The National Academy of Sciences of the USA

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Fig. 4. — Band shifts due to an increased PrP^C concentration at the membrane. A decrease in the second derivative of a spectrum refers to an actual increase of extinction in the absorbance band. (A and B) The amide I bands, as recorded during membrane anchoring, were normalized to identical amplitudes at 1,642 cm⁻¹. (C) Band shifts were identified at 1,680 and 1,642 cm⁻¹ for 1.0 μM PrP^C. (D) The according analysis of the 0.4 μM assay indicated an earliest onset of the discussed structural conversion after 34 min incubation.