Fig. 3.

The spectrum of the kinetic component (the P_R → O transition) of the oxygen reaction in the ATR sample of the Glu-278–Gln mutant enzyme obtained in the reflectance mode on a millisecond time scale. Conditions were: K-phosphate buffer, 200 mM (pH 6); hexaamineruthenium, 10–20 μM; glucose, 100 mM; catalase, 260 mg/ml; glucose oxidase, 670 mg/ml; K-ascorbate, 3.3 mM; CO, 100%. The reaction was started by a laser flash ≈350 ms after the beginning of the injection of 100 μl of oxygen-saturated buffer.