Fig. 1.

The ternary complex interacting with the decoding center of the A-site as seen by using cryo-EM. (A) The ribosome position of the ternary complex during the decoding process (A/T-site). (B–D) Fitting the aminoacyl-tRNA within the ribosomal-bound ternary complex. To satisfactorily fit the crystal structure of a tRNA into the corresponding cryo-EM density requires the introduction of a kink ≈40° in the anticodon stem, just below the anticodon stem of the aminoacyl-tRNA. (According to ref. 23, modified.)