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Labeling of γ-secretase within the proteoliposomes. A, Western blot of purified γ-secretase run on a 4–20% Tris glycine gel and probed with γ-secretase antibodies specific for NCT-GST, PS1-NTF, APH1α2-HA, PS1-CTF, and FLAG-Pen-2, before in vitro biotinylation (lane 1) and then probed with anti-biotin after biotinylation (lane 2). B, immunogold EM of proteoliposomes probed with anti-biotin antibody plus 10-nm conjugated Protein A-gold, and counterstained with uranyl acetate.
