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Characterization of Bla g 2 and its mutants. A, CD spectra of rBla g 2 and mutant proteins Mut 1 and Mut 2. B, comparison of the rBla g 2 mutant proteins and rBla g 2 wild-type (N1G2) by ELISA. C, size-exclusion chromatography of Bla g 2 wild type, Mut 1 and Mut 2.
