ERK, MAPK, phosphoinositide 3-kinase, and NF-κB signaling pathways
are involved in the 2-AG-induced suppression of COX-2 elevation.
a1, Western immunoblot analysis of phosphorylation of p38 MAPK in
hippocampal tissue from mice that received vehicle, LPS, LPS + 2-AG, and LPS +
2-AG + SR-1. a2, quantifications of p38 MAPK phosphorylation.
b1, immunoblot analysis of NF-κB phosphorylation in the mouse
hippocampus. b2, quantifications of NF-κB phosphorylation
(n = 3). **, p < 0.01 compared with vehicle
controls; ##, p < 0.01 compared with LPS. c1, Western
blot analysis of ERK1/2 and Akt phosphorylation in hippocampal tissue from
mice injected with vehicle, LPS, and LPS + 2-AG. c2 and c3,
quantifications of ERK1/2 and Akt phosphorylation. **, p
< 0.01 compared with vehicle controls; ##, p < 0.01 compared
with LPS (n = 3). d1, Western blot analysis of ERK1/2 and
Akt phosphorylation in hippocampal tissue from mice injected with vehicle, KA,
KA + 2-AG, and KA + 2-AG + SR-1. c2 and c3, quantifications
of ERK1/2 and Akt phosphorylation. **, p < 0.01
compared with vehicle controls; ##, p < 0.01 compared with KA
(n = 3).