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SPARC is increased in WT LEC during cellular stress. WT LEC were either serum-deprived (48 h) or exposed to 5 μg/ml tunicamycin (24 h). Protein from total cell lysates (20 μg/lane) and conditioned media (volume determined by relative protein concentration from lysates) were immunoblotted for SPARC, ILK, and integrin β1. Composite blots are from single membranes and are representative of three independent experiments. GAPDH was probed as a loading control.
