FIGURE 9.

Identification of epitopes recognized by anti-SPARC mAbs. A Multipin™ peptide array representing the full sequence of hSPARC as contiguous and overlapping 10-mers (see “Experimental Procedures”) was used to identify epitopes recognized by 4 anti-SPARC mAbs. Each graph (A-D) shows the reactivity of the mAb against overlapping, sequential peptides in three separate experiments. The inset panel displays predicted epitope position (red) in a molecular model of SPARC derived from A chain coordinates of Protein Bank Accession 1BMO (40). The blue bar along the x axis denotes the sequence represented in the molecular model. A, anti-SPARC mAb 236. B, anti-SPARC mAb 255. C, anti-SPARC mAb 293. D, anti-SPARC mAb 303. Potential epitopes were selected on the basis of high reactivities with contiguous peptides, and predicted epitopes are designated by red rectangles.