FIGURE 7.

Disruption of the interaction of mutant SOD1 with dynein affects cell viability. Cell viability in the absence and presence of p50 overexpression was determined by the percentage of PI-positive transfected cells. A, representative images show PI-positive HEK293 cells expressing WT or A4V SOD1 in the absence of p50 overexpression. B, quantification of cell viability obtained from three independent experiments. The black bars are the percentage of PI-positive cells without inclusions, and the gray bars are the percentage of PI-positive inclusion-containing cells. Standard deviations shown are for the total number of PI-positive cells. ^*, p < 0.05 and ^**, p < 0.03.