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The C/EBPβ mRNA associates with heavy polysomes during prolonged ER stress. C6 cells were treated with Tg for the indicated times, and cytoplasmic extracts were analyzed by sucrose gradient centrifugation as described under “Experimental Procedures.” Total RNA samples were run on agarose gels to analyze ribosomal RNAs (upper panels). qRT-PCR was used to monitor the distribution of C/EBPβ, tubulin, and ATF mRNAs (lower panels). CON, control.
