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. 2008 Aug 15;283(33):22443–22456. doi: 10.1074/jbc.M801046200

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FIGURE 7. — Induction of stress-response proteins in C/EBPβ^+/+, C/EBPβ^–/–, and C/EBPβ^–/– (LIP) MEFs. WT, mutant, and C/EBPβ^–/– cells stably expressing LIP (C/EBPβ^–/– (LIP)) were treated with Tg for the indicated times. A and C–E, Western blot analysis of nuclear (A) or total (C–E) cell extracts probed with antibodies for the indicated proteins. B, quantification of the LIP/LAP ratio from A. F, quantification of C/EBPβ mRNA levels in MEFs treated with Tg for the indicated times. Data from qRT/PCR analysis of total RNA using C/EBPβ specific primers were normalized to the signal of 18 S ribosomal RNA. CON, control.