FIGURE 10.
Phosphorylation of ΔNMBD protein with Pi and subsequent decay. A, phosphorylation was started by the addition of 50 μm [32P]Pi to ΔNMBD in conjunction with either 1 mm BCS (•) or 40 μm CuCl2 (○), or following a 2-min preincubation with 40 μm CuCl2 (▾). B, decay was started with a 1 mm non-radioactive Pi chase added to [32P]phosphoenzyme obtained by a 5-min equilibration with 50 μm [32P]Pi in the absence of both BCS and CuCl2. The chase medium contained either 1 mm BCS (•) or 40 μm Cu2+ (○). The temperature was 60 °C.