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Phosphorylation of ΔNMBD (A) and CXXC(B) by utilization of ATP. The reaction was started by addition of 25 μm [γ-32P]ATP to ΔNMBD (A) or CXXC(B). The protein was preincubated with either 1 mm BCS (○) or 40 μm CuCl2 (•). See “Materials and Methods” for reaction mixtures. The temperature was 60 °C.
