FIGURE 12.

Tonicity-responsive nuclear trafficking of GFP trimer (3GFP) by the NLS of TonEBP. A, schematics of recombinant constructs. 3GFP is an in-frame trimer of GFP. The Myc-SV40NLS fragment shown in Fig. 8A was used to produce 3GFP-SV40NLS. SV40NLS in 3GFP-SV40NLS was replaced by the TonEBP fragment 181–234 to produce 3GFP-NLS. B, COS7 cells transfected with pEGFP-C2 encoding a monomeric GFP or the 3GFP constructs were immunoblotted (IB) for GFP. C, the transfected cells were analyzed as in Fig. 3 using the green fluorescence. Representative fluorescence images in hypotonic, isotonic, and hypertonic conditions are shown at the bottom.