FIGURE 2.

Nuclear import of TonEBP in response to hypertonicity. A, dose response. COS7 cells had been incubated for 30 min in hypotonic medium (135 mosmol/kg) before the medium osmolality was increased up to 335 mosmol/kg, as indicated by the addition of NaCl. After 30 min, TonEBP was visualized by immunofluorescence. B, time course. Cells had been incubated for 30 min in hypotonic medium before they were switched to isotonic medium for 5, 15, or 30 min. C, cells grown on plastic dishes were treated as in B except that they were switched to isotonic medium for up to 120 min. TonEBP and HSC70 were detected by immunoblotting (IB).