FIGURE 5.

Nuclear export of TonEBP is insensitive to LMB. A, COS7 cells grown on glass coverslips were treated with vehicle or 10 ng/ml LMB for 30 min and then switched to hypotonic medium or kept in isotonic medium for another 30 min as indicated. TonEBP was visualized by immunofluorescence. B, COS7 cells transfected with hemagglutinin-tagged NFATc1 were seeded on glass coverslips. The cells were treated with 10 ng/ml ionomycin for 30 min in the absence or presence of LMB. They were then switched to medium without ionomycin for 30 min, followed by immunofluorescence visualization of the hemagglutinin tag.