FIGURE 6.

Putative nuclear export signals of TonEBP. A, schematics of TonEBP and its mutant constructs made by deletion and site-directed mutagenesis. Amino acid sequences are shown for pnes1 (residues 8–15) and pnes2 (residues 146–156). TonEBP-a is the a-form of the TonEBP splice variant. B, an immunoblot (IB) of COS7 cells transfected with the constructs in fusion with GFP is shown below. The transfected cells were analyzed as in Fig. 3, and results from cells in hypotonic and isotonic conditions are shown (mean ± S.D., n = 4). In hypertonic conditions, the distributions were identical in all constructs (not shown) (i.e. the same as TonEBP).