BCL6 triggers a p53 response and growth arrest in WI-38 cells.
A, quantitative PCR was performed in WI-38 fibroblasts 48 h after
transduction as indicated to determine the -fold change in transcript
abundance for ATM, TP53, and P21, expressed relative to
untransduced cells. B, Western blot was performed to detect the
amount of p53 protein, as compared with actin loading control. C, the
percent of cells in G1, S, and G2/M phase was determined
by propidium iodide FACS. D, the proliferation of WI-38 cells was
assessed over a 5-day time course at the indicated time points. Ctrl
refers to untransduced WI-38 cells.