FIGURE 3.

BCL6 triggers a p53 response and growth arrest in WI-38 cells. A, quantitative PCR was performed in WI-38 fibroblasts 48 h after transduction as indicated to determine the -fold change in transcript abundance for ATM, TP53, and P21, expressed relative to untransduced cells. B, Western blot was performed to detect the amount of p53 protein, as compared with actin loading control. C, the percent of cells in G₁, S, and G₂/M phase was determined by propidium iodide FACS. D, the proliferation of WI-38 cells was assessed over a 5-day time course at the indicated time points. Ctrl refers to untransduced WI-38 cells.