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. 2008 Aug 15;283(33):22565–22572. doi: 10.1074/jbc.M803490200

FIGURE 5.

FIGURE 5.

Induction of senescence in fibroblasts is dependent on p53. A and B, the proliferation of Tp53+/+ and Tp53-/- MEFs, respectively, transduced as indicated was measured over an 8-day period. C and D, quantification of percent Tp53+/+ and Tp53-/- MEFs cells, respectively, positive for acid β-galactosidase activity (i.e. senescent cells) after 10 days. E, representative photomicrographs of MEFs from panel B stained for acid β-galactosidase activity. F, Western blots were performed to determine protein abundance of BCL6 and p53 in wild type MEFs transduced as indicated. G, microscopic images of Tp53-/- MEFs transduced as indicated after 10 days. Upper panels are phase-contrast and lower panels are fluorescence microscopy to determine growth patterns of transduced cells as indicated after 10 days. Ctrl refers to untransduced MEFs.