Induction of senescence in fibroblasts is dependent on p53.
A and B, the proliferation of Tp53+/+
and Tp53-/- MEFs, respectively, transduced as indicated
was measured over an 8-day period. C and D, quantification
of percent Tp53+/+ and Tp53-/- MEFs
cells, respectively, positive for acid β-galactosidase activity
(i.e. senescent cells) after 10 days. E, representative
photomicrographs of MEFs from panel B stained for acid
β-galactosidase activity. F, Western blots were performed to
determine protein abundance of BCL6 and p53 in wild type MEFs transduced as
indicated. G, microscopic images of Tp53-/- MEFs
transduced as indicated after 10 days. Upper panels are
phase-contrast and lower panels are fluorescence microscopy to
determine growth patterns of transduced cells as indicated after 10 days.
Ctrl refers to untransduced MEFs.