FIGURE 7.

BCL6 causes misrepair and deletion of double-strand breaks. Cellular extracts were obtained from naïve B-cells transduced with BCL6, GFP, or mock transduced. A linearized LacZ containing plasmid was incubated in these extracts, and then recovered and analyzed by blue/white colony forming assays and PCR. The left panel shows the percent of colonies that have lost the ability to express functional LacZ, which has doubled in the BCL6 expressing cells. The right panel indicates the percentage of recovered plasmids that have undergone extensive deletions, which again has doubled in the presence of BCL6. Ctrl refers to untransduced naïve B-cells.