FIGURE 3.

Knockdown of endogenous DEC1 partially attenuates the inhibition of ID1 expression upon DNA damage. Western blots were prepared with extracts from HCT116 cells that were transiently transfected with control scramble siRNA and DEC1 siRNA for 3 days, and then untreated (–) or treated (+) with doxorubicin (Dox) (0.3 μg/ml) for 24 h. The blots were analyzed as described in the legend to Fig. 1A.