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. 2008 Aug 15;283(33):22417–22429. doi: 10.1074/jbc.M710415200

FIGURE 2.

FIGURE 2.

Cell surface association of soluble HBD2. A, HBD2-specific ELISA analysis of medium supernatant and cell lysate collected from RSV-infected (0–16 h post-infection) A549 cells. B, HBD2-specific ELISA analysis of medium supernatant and cell lysate collected from TNF-treated (0–16 h treatment) A549 cells. C, medium supernatant and cell lysate collected from A549 cells incubated with 1 μg of purified HBD2 for 0–16 h were subjected to ELISA to detect cell-associated versus soluble HBD2. Amount of HBD2 deduced from the ELISA was expressed as ng/ml, and each value represents the mean ± S.D. for three determinations. D, A549 cells incubated with 1 μg of purified HBD2 for 0–24 h were biotinylated (cell surface biotinylation), followed by precipitation with avidin-agarose and blotting with anti-HBD2 antibody.