Table 1.
B lymphocyte transformation by EBV recombinants
| Original LCL source of virus | Viral genomes in original LCLs | % infected wells with LCLs | Number of resultant LCLs with:
|
||
|---|---|---|---|---|---|
| F-LMP1 alone | F-LMP1 & P3HR-1 LMP1 | P3HR-1 LMP1 alone | |||
| F-LMP1-1 | F-LMP1 & P3HR-1 | 17 | 8 | 7 | 16 |
| 1 | 1 | 0 | 1 | ||
| F-LMP1-2 | F-LMP1 & P3HR-1 | 9 | 17 | 0 | 0 |
| 1 | 1 | 0 | 0 | ||
| F-LMP1-ID-1 | F-LMP1-ID & P3HR-1 | 19 | 0 | 86 | 8 |
| 2 | 0 | 19 | 2 | ||
| F-LMP1-ID-2 | F-LMP1-ID & P3HR-1 | 100 | 0 | 10 | 85 |
| 5 | 0 | 1 | 19 | ||
| F-LMP1-ID-3 | F-LMP1-ID & P3HR-1 | 11 | 0 | 0 | 14 |
| F-LMP1-ID-4 | F-LMP1-ID & P3HR-1 | 15 | 0 | 0 | 29 |
| F-LMP1-FFD-1 | F-LMP1-FFD | 38 | 48 | 0 | 0 |
Virus replication was induced in EBV-infected LCLs (column 1). B lymphocytes were infected with dilutions of the resultant viruses (column 2) and seeded into 96-well plates. If the percentage of wells with LCLs (column 3) is less than 40%, P3HR-1 usually is segregated from a transforming recombinant. LCLs that are infected with a recombinant alone (column 4) are positive for F-LMP1 DNA and negative for P3HR-1 LMP1 DNA. LCLs coinfected with a recombinant and P3HR-1 (column 5) are positive for both F-LMP1 and P3HR-1 LMP1 DNA. Secondary recombinant transformed LCLs (column 6) are infected with a transforming EBV that has only P3HR-1 LMP1 DNA.