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Deacetylation rate of Tyr-411. At time 0, Tyr-411 was acetylated 80%. After 61 h at pH 8.0, 22 °C, Tyr-411 was acetylated 40%. Deacetylation was monitored for 3.5 half-lives until only 5% of Tyr-411 was still acetylated. The three horizontal lines mark 3 half-lives. % acetylation was calculated from cluster areas in the MALDI-TOF mass spectrometer for peptides VRYTKKVPQVSTPTL and LVRYTKKVPQVSTPTL.
