Table 1.
Effect of the association promoter/host strain on the production of the active recombinant US132 CGTase.
Plasmids (promoters) | pSJ8 (T7) | pSJ9 (Trc) | |||
---|---|---|---|---|---|
E. coli strains | DH5α | ER2566 | DH5α | ER2566 | JM109 |
Enzyme production (U/mL) | 0.32 ± 0.08 | 0.92 ± 0.08 | 0.35 ± 0.05 | 1.07 ± 0.03 | 0.85 ± 0.05 |