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Purification steps of the recombinant US132 CGTase.
| Purification step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
|---|---|---|---|---|---|
| Crude extract | 7000 | 35 | 200 | 100 | — |
| Heat treatment | 6650 | 14.8 | 450 | 95 | 2.2 |
| HIC* | 4340 | 1.55 | 2800 | 62 | 14 |
| Starch adsorption | 1400 | 0.28 | 5000 | 20 | 25 |
*HIC: Hydrophobic Interaction Chromatography.
