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Effect of preinjection of empty RCR on gene delivery by DNA/TC-Chol-RCR. Fifteen minutes after intraportal injection of 0.6 ml of isotonic mannitol with or without empty RCR (5.0 mg of total lipids), mice were intraportally injected with 50 μg DNA of pCMVL/TC-Chol-RCR dispersed in 0.6 ml of isotonic mannitol. Two days after injection, mice were sacrificed and luciferase activity in the various organ extracts was measured. Each column presents the mean from three animals ± SD.
