Skip to main content
. Author manuscript; available in PMC: 2008 Aug 11.
Published in final edited form as: Biochemistry. 2006 Mar 14;45(10):3357–3369. doi: 10.1021/bi052437v

Figure 7.

Figure 7

Effect of β-elimination and Michael addition with cysteamine on T55 and related peaks. A. The oatp1a1 tryptic digest was preoxidized with H2O2 prior to obtaining this spectrum. B. The preoxidized peptides were subjected to β-elimination with barium and sodium hydroxide. The single asterisk indicates the β-eliminated pT54T55, while the double asterisk indicates the β-eliminated ppT54T55. C. The β-eliminated peptides were subjected to Michael addition with cysteamine. The single asterisk indicates the cysteamine modified product of pT54T55, while the double asterisk indicates the cysteamine modified product of ppT54T55.