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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: J Struct Biol. 2008 May 6;163(1):29–39. doi: 10.1016/j.jsb.2008.04.005

Fig. 5.

Fig. 5

Variations between datasets and the influence of dose on resolution. Panels A-E are for data collected at 120keV and F-J are for data collected at 200keV. A) Plot of resolution as a function of number of particles for data collected at 120keV and 100,000X. Two datasets were acquired with a dose of 13e-/Å2 (blue curves) while the third was acquired with a dose of 19e-/Å2 (red curve). Resolution was estimated by the FSC0.5 criterion. B) Same as A, but resolution was estimated by rmeasure. C,D) The highest-resolution 3D reconstructions for the data collected with a dose of 13e-/Å2. E) The highest-resolution 3D reconstructions for the data collected with a dose of 19e-/Å2. F) Plot of resolution as a function of number of particles for data collected at 200keV and 100,000X. Two datasets were acquired with a dose of 13e-/Å2 (blue curves) while the third was acquired with a dose of 19e-/Å2 (red curve). Resolution was estimated by the FSC0.5 criterion. G) Same as A, but resolution was estimated by rmeasure. H,I) The highest-resolution 3D reconstructions for the data collected with a dose of 13e-/Å2. J) The highest-resolution 3D reconstructions for the data collected with a dose of 19e-/Å2.