Figure 2.

Northern blot analysis confirming differential expression of a specific mRNA in VIIa-treated cells. WI-38 monolayers were treated with the control buffer or the buffer containing VIIa, 10 nM, for 90 min at 37°C. Total RNA was extracted from the cells and 15 μg of each RNA sample was used for Northern blot analysis. The blot was hybridized with a cDNA fragment of band 11 isolated from the display gel of Fig. 1C. (Upper) Northern blot analysis with band 11 cDNA. (Lower) Ethidium bromide staining of total RNA samples as controls for equal loading.