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. Author manuscript; available in PMC: 2009 Aug 8.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Jun 2;372(4):811–815. doi: 10.1016/j.bbrc.2008.05.121

Fig. 2.

Fig. 2

Detection of symmetric dimethylarginine (SDMA) as a minor product in histone H2A incubated with Hsl7 for extended times. Amino acid analysis of histone H2A methylation in wild type GST-Hsl7 (panel A) and mutant GST-Hsl7G387A (panel B) incubations for 20 h at 37 °C as described in “Materials and methods” and in the legend to Fig. 1. Controls are also shown where GST-Hsl7 (panel C) and GST-Hsl7G387A (panel D) were incubated under similar conditions in the absence of histone H2A. 3H-Radioactivity (cpm) is seen in the black line with diamonds corresponding to every fraction of the 1 ml/min elution of the cation exchange column. The ninhydrin peaks of unlabeled ω-MMA, SDMA, and ADMA are seen by gray lines with squares at 570 nm absorption corresponding to every fraction as well.