Cells plated in 6-well plates were treated with the indicated concentrations of PDNF or NGF for 48 h in DMEM/FCS (1%).
(A) ChAT and VAChT expression analyzed by RT-PCR and by
(B) q PCR. β-actin expression was used as an internal control.
Values given are fold difference between treated samples and untreated controls. Error bars represent SEM (n=3), * - P < 0.05, ** - P < 0.01.