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. Author manuscript; available in PMC: 2009 Jun 27.
Published in final edited form as: Brain Res. 2008 Apr 10;1217:195–202. doi: 10.1016/j.brainres.2008.03.082

Figure 3. PDNF increases number of ChAT-expressing PC12 cells.

Figure 3

Cells were treated as in Fig. 1 with or without 250 ng/ml PDNF or 100 ng/ml NGF, fixed in paraformaldehyde, permeabilized with a non-ionic detergent, ChAT visualized with specific antibody (AB144P) followed by a fluorescent-labeled secondary antibody (green), and nuclear DNA with DAPI (blue).