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. Author manuscript; available in PMC: 2009 Jun 27.
Published in final edited form as: Brain Res. 2008 Apr 10;1217:195–202. doi: 10.1016/j.brainres.2008.03.082

Figure 5. Cholinergic gene expression is down-regulated in PC12 cells with intracellular T. cruzi infection and up-regulated by extracellular T. cruzi trypomastigotes.

Figure 5

(A) PC12 cells were infected with 1.0 × 106 trypomastigotes for 3, 24 and 48 h, cells harvested and analyzed for ChAT, VAChT, and β-actin expression by RT-PCR (a) and qPCR (b). Error bars represent SEM (n=4), * - P < 0.05, ** - P < 0.01, ns – non-significant.

(B) PC12 cells were exposed to heat-treated trypomastigotes (106/ml) or NGF (100 ng/ml) for 48 h, and ChAT, VAChT transcripts determined by RT-PCR. Graph represents quantitation of the response determined by scanning densitometry.