(A) PC12 cells were infected with 1.0 × 106 trypomastigotes for 3, 24 and 48 h, cells harvested and analyzed for ChAT, VAChT, and β-actin expression by RT-PCR (a) and qPCR (b). Error bars represent SEM (n=4), * - P < 0.05, ** - P < 0.01, ns – non-significant.
(B) PC12 cells were exposed to heat-treated trypomastigotes (106/ml) or NGF (100 ng/ml) for 48 h, and ChAT, VAChT transcripts determined by RT-PCR. Graph represents quantitation of the response determined by scanning densitometry.