Figure 4.

Detection and identification of a 9−48 disulfide bridge in hTf/2N by broadband CAD of anions (m/z 1090−1150) derived from digestion of this protein with trypsin. The broadband spectra acquired below and above the fragmentation threshold are shown in gray and black, respectively. The inset shows a group of peaks corresponding to peptide [8−18]. Anionic species representing another peptide from the dimer, [43−50], are marked with squares.