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Detection and identification of a 137−331 disulfide bridge in hTf/2N by broadband CAD of anions (m/z 1550−1620) derived from digestion of this protein with trypsin. The broadband spectra acquired below and above the fragmentation threshold are shown in gray and black, respectively. The inset shows a group of peaks corresponding to peptide [125−143]. Anionic species representing another peptide from the dimer, [328−337], are marked with squares.
