Table 2.

Relative activities of pig heart KGDHC in the presence of various GSTQ_n constructs with or without added tTGase

Addition	Concentration (mg/ml)	Relative Activities (%)*
		−tTGase	+tTGase
None (control)	–	103  ± 2	[100  ± 3]
GSTQ0	1	99  ± 3	107  ± 7
GSTQ10	1	100  ± 2	95  ± 9
GSTQ62	0.3	98  ± 5	59  ± 9†§
GSTQ81‡	0.3	102  ± 3	70  ± 2†§
GSTA61 (control)	1.2	95  ± 6	101  ± 3

^*Relative activity remaining after 3 hr at 20°C. 

^†Significantly different from the control (i.e., a reaction mixture lacking GST construct but containing tTGase) with P = 0.05. 

^‡A sonicated preparation of the GST construct was used in this experiment. 

^§Relative activities at 1 and 2 hr were 85 ± 5 and 70 ± 6%, respectively, in the presence of GSTQ₆₂ plus tTGase, and 88 ± 3 and 82 ± 4%, respectively in the presence of GSTQ₈₁ plus tTGase.