Figure 3.

Overexpression of XGPR3 in Xenopus Oocytes Inhibited Maturation

A and B, Overexpression of XGPR3 markedly inhibited testosterone-mediated oocyte maturation (A) and activation of MAPK and CDK1 (B) in denuded oocytes. Maturation was determined by examining oocytes for Germinal Vesicle Breakdown (GVBD) after overnight incubation with testosterone. After 6 h with testosterone, MAPK activation was detected by Western blot for increased phosphorylation of p42, whereas CDK1 activation was determined by Western blot for decreased phosphorylation of CDK1. Total p42 and MAPK expression in the same samples are also shown. 100T, 100 nm testosterone; 1000T, 1000 nm testosterone. C, Overexpression of XGPR3 markedly inhibited hCG (150 Units/ml)- and testosterone (500 nm)-induced oocyte maturation in follicle-enclosed oocytes. All studies were performed at least three times with similar results.