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. 2008 May 29;22(8):1950–1961. doi: 10.1210/me.2008-0127

Figure 2.

Figure 2

Activation of CB1R Decreases Action Potential Frequency in SF-1 Neurons

A, Sample traces from a SF-1/eGFP-positive neuron recorded before, during, and after application of the CB1R agonist WIN 55212-2 (1 μm) in current clamp configuration. Treatment with WIN 55212-2 markedly decreased action potential frequency of SF-1 neuron. (Top panel: scale bar: 50 mV, 2 min). The bottom panel shows activity of the same SF-1 neuron on an expanded time scale before and immediately after application of WIN 55212-2. Scale bar: 50 mV, 2 sec. Vm = −45 mV. B, Sample traces from a SF-1/eGFP-negative VMH neuron recorded before and after application of WIN 55212-2 (1 μm) in current clamp configuration. The CB1R agonist did not alter the firing rate of SF-1/eGFP-negative neurons. Scale bar: 50 mV, 0.5 sec. Vm = −50 mV. C, Pooled data of the frequency of action potentials in SF-1/eGFP-positive and -negative VMH neurons before and after treatment with WIN 55212-2 (9 and 7 neurons, respectively; *, P < 0.05). Error bars represent sem.