Figure 4.

SF-1 Directly Regulates CB1R Expression

A, Schematic diagram of the 5′-flanking region of the mouse Cb1r gene, including the location of potential SF-1 binding sites. Shown below are the 5′-deletion constructs. B, CB1R promoter activity is significantly stimulated by SF-1 in mouse Y1 adrenocortical cells and human HEK 293 kidney cells in a dose-dependent manner (*, P < 0.001, t test). C, 5′-Deletion analysis of SF-1 binding sites that regulate the CB1R promoter. The 293 cells were cotransfected with SF-1 and the deletion constructs shown in Fig. 4A. Deletion of putative SF-1 binding sites in the −246 and −108 constructs significantly decreased promoter activity (**, P < 0.001, one-way ANOVA).